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pcmv myc vector  (TaKaRa)


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    TaKaRa pcmv myc vector
    Pcmv Myc Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 758 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv+myc+vector/pCMV-Myc+Vector+Set/pmc13179329-189-59-61
    Average 96 stars, based on 758 article reviews
    pcmv myc vector - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Synthesized:

    Article Title: A nanobody-based proteolysis-targeting chimera offers broad-spectrum protection against diverse influenza virus infections
    Article Snippet: Puromycin (HY-B1743), LY294002 (HY-10108), and MG132 (HY-13259) were purchased from MedChemExpress. .. The genes encoding the α-domain of the human von Hippel‒Lindau (VHL) tumor suppressor (GenBank accession no. NP_000542.1 ), mouse VHL (GenBank accession no. NP_033533.1 ), and anti-NP nanobodies (Nb52, Nb77, Nb135, Nb170, and Nb355) and an anti-SARS-CoV-2 Spike nanobody (GenBank accession no. QNA42481.1 ) were synthesized and cloned into the lentiviral expression vector pLVX-CMV-Puromycin (Addgene plasmid #164589) and/or the pCMV-Myc vector (Clontech, 635689). ..

    Clone Assay:

    Article Title: A nanobody-based proteolysis-targeting chimera offers broad-spectrum protection against diverse influenza virus infections
    Article Snippet: Puromycin (HY-B1743), LY294002 (HY-10108), and MG132 (HY-13259) were purchased from MedChemExpress. .. The genes encoding the α-domain of the human von Hippel‒Lindau (VHL) tumor suppressor (GenBank accession no. NP_000542.1 ), mouse VHL (GenBank accession no. NP_033533.1 ), and anti-NP nanobodies (Nb52, Nb77, Nb135, Nb170, and Nb355) and an anti-SARS-CoV-2 Spike nanobody (GenBank accession no. QNA42481.1 ) were synthesized and cloned into the lentiviral expression vector pLVX-CMV-Puromycin (Addgene plasmid #164589) and/or the pCMV-Myc vector (Clontech, 635689). ..

    Article Title: KCTD1 stabilizes c-Myc to upregulate PD-L1 and suppress anti-tumor immunity in hepatocellular carcinoma.
    Article Snippet: .. Full-length and truncated human c-Myc fragments including N-terminal (1–143 aa), central (144– 354 aa), and C-terminal (355–439 aa) were PCR-amplified and cloned into the pCMV-Myc vector (Clontech). ..

    Article Title: KCTD1 stabilizes c-Myc to upregulate PD-L1 and suppress anti-tumor immunity in hepatocellular carcinoma
    Article Snippet: .. Full-length and truncated human c-Myc fragments including N-terminal (1–143 aa), central (144–354 aa), and C-terminal (355–439 aa) were PCR-amplified and cloned into the pCMV-Myc vector (Clontech). ..

    Article Title: MOV10 inhibits SADS-CoV replication by enhancing TRIM24-mediated K63-linked TRAF3 ubiquitination and this inhibition is antagonized by viral N protein
    Article Snippet: .. SADS-CoV N protein ( GenBank accession number: ON911569.1 ) was cloned into the pAcGFP-C1 (632470; Clontech) and pCMV-Myc vector. ..

    Expressing:

    Article Title: A nanobody-based proteolysis-targeting chimera offers broad-spectrum protection against diverse influenza virus infections
    Article Snippet: Puromycin (HY-B1743), LY294002 (HY-10108), and MG132 (HY-13259) were purchased from MedChemExpress. .. The genes encoding the α-domain of the human von Hippel‒Lindau (VHL) tumor suppressor (GenBank accession no. NP_000542.1 ), mouse VHL (GenBank accession no. NP_033533.1 ), and anti-NP nanobodies (Nb52, Nb77, Nb135, Nb170, and Nb355) and an anti-SARS-CoV-2 Spike nanobody (GenBank accession no. QNA42481.1 ) were synthesized and cloned into the lentiviral expression vector pLVX-CMV-Puromycin (Addgene plasmid #164589) and/or the pCMV-Myc vector (Clontech, 635689). ..

    Plasmid Preparation:

    Article Title: A nanobody-based proteolysis-targeting chimera offers broad-spectrum protection against diverse influenza virus infections
    Article Snippet: Puromycin (HY-B1743), LY294002 (HY-10108), and MG132 (HY-13259) were purchased from MedChemExpress. .. The genes encoding the α-domain of the human von Hippel‒Lindau (VHL) tumor suppressor (GenBank accession no. NP_000542.1 ), mouse VHL (GenBank accession no. NP_033533.1 ), and anti-NP nanobodies (Nb52, Nb77, Nb135, Nb170, and Nb355) and an anti-SARS-CoV-2 Spike nanobody (GenBank accession no. QNA42481.1 ) were synthesized and cloned into the lentiviral expression vector pLVX-CMV-Puromycin (Addgene plasmid #164589) and/or the pCMV-Myc vector (Clontech, 635689). ..

    Article Title: KCTD1 stabilizes c-Myc to upregulate PD-L1 and suppress anti-tumor immunity in hepatocellular carcinoma.
    Article Snippet: .. Full-length and truncated human c-Myc fragments including N-terminal (1–143 aa), central (144– 354 aa), and C-terminal (355–439 aa) were PCR-amplified and cloned into the pCMV-Myc vector (Clontech). ..

    Article Title: Parkin-mediated ubiquitination of hif-α modulates hypoxia signaling in Megalobrama amblycephala.
    Article Snippet: .. Following ligation of the amplified parkin gene fragment into the pCMV-Flag and pCMV-Myc vector (Clontech), the Flag-Ma-parkin and Myc-Ma-parkin fusion protein expression systems were constructed. .. AR TIC LE IN PR ES S 2.5 Quantitative real-time PCR (qRT-PCR) Analysis Phenol/chloroform-based RNA extraction from biological samples used TransZol reagent (TransGen Biotech).

    Article Title: KCTD1 stabilizes c-Myc to upregulate PD-L1 and suppress anti-tumor immunity in hepatocellular carcinoma
    Article Snippet: .. Full-length and truncated human c-Myc fragments including N-terminal (1–143 aa), central (144–354 aa), and C-terminal (355–439 aa) were PCR-amplified and cloned into the pCMV-Myc vector (Clontech). ..

    Article Title: A selective RPL15 PROTAC degrader enhances anti-PD-1 immunotherapy in a murine melanoma tumor model
    Article Snippet: The following antibodies were used for immunoblotting: anti-Myc antibody (M4439, 9E10, ×1000 dilution, Sigma), anti-HA antibody (#3724, C29F4, ×1000 dilution, Cell Signaling Technology, Danvers, MA, USA), anti-FLAG antibody (F3165, M2, ×1000 dilution, Sigma), anti-CRBN (#71810, D8H3S, ×1000 dilution, CST), anti-Histone H3 (sc-517576, 1G1, ×500 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), anti-β-actin antibody (sc-69879, AC-15, ×2000 dilution, Santa Cruz Biotechnology), anti-eIF2α antibody (#9722, ×1000 dilution,Cell Signaling Technology), anti-phospho-eIF2α S51 antibody (#3398, D9G8, ×1000 dilution, Cell Signaling Technology), anti-JNK1/2 antibody (#9252, ×1000 dilution, Cell Signaling Technology), anti-phospho-JNK1/2 antibody T183/Y185 (#9251, ×1000 dilution, Cell Signaling Technology), anti-ZAK antibody (A301-994A, ×1000 dilution, Bethyl Laboratories, Waltham, MA, USA), anti-RPL15 antibody (VPA00489KT, ×1000 dilution, Bio-Rad Laboratories, Hercules, CA, USA), anti-RPS3 antibody (#9538, D50G7, ×1000 dilution, CST), anti-p53 antibody (sc-6243, ×500 dilution, Santacruz), anti-p21 antibody (F-5, sc-6246, ×300 dilution, Santacruz), anti-TOP1 antibody (EPR5375, ×1000 dilution, Abcam, Cambridge, UK), anti-mouse IgG-HRP antibody (NA931, ×9000 dilution, Cytiva, Tokyo, Japan), and anti-rabbit IgG-HRP antibody (NA934, ×9000 dilution, Cytiva). .. RPL15 cDNA was amplified from HeLa cells' cDNA by PCR and inserted into the pCMV-Myc vector (Takara Bio Inc., Shiga, Japan). .. For the construction of the lentiviral expression vector of human CRBN, Flag-tagged CRBN cDNA was amplified from HeLa cells' cDNA by PCR using the primer pairs containing FLAG sequences and inserted into the plenti-CAG-IRES-EGFP vector (#69047, Addgene).

    Article Title: Parkin-mediated ubiquitination of hif-α modulates hypoxia signaling in Megalobrama amblycephala
    Article Snippet: .. Following ligation of the amplified parkin gene fragment into the pCMV-Flag and pCMV-Myc vector (Clontech), the Flag- Ma-parkin and Myc- Ma-parkin fusion protein expression systems were constructed. .. Phenol/chloroform-based RNA extraction from biological samples used TransZol reagent (TransGen Biotech).

    Article Title: Seneca Valley virus 2B and 3 C proteins attenuate the cGAS-STING signaling pathway by targeting STING for degradation.
    Article Snippet: Seneca Valley virus (SVV), an emerging porcine picornavirus, has attracted significant attention recently as it presents a substantial threat to the global pork industry.. Stimulator of interferon genes (STING) is an endoplasmic reticulum (ER)-associated adaptor protein that plays a crucial role in the type I interferon (IFN-I) signaling pathway, which triggers IFN-stimulated gene (ISG) production to elicit antiviral innate immunity.. SVV has evolved numerous strategies to counteract the IFN-I signaling pathway and evade innate immunity.

    Article Title: MOV10 inhibits SADS-CoV replication by enhancing TRIM24-mediated K63-linked TRAF3 ubiquitination and this inhibition is antagonized by viral N protein
    Article Snippet: .. SADS-CoV N protein ( GenBank accession number: ON911569.1 ) was cloned into the pAcGFP-C1 (632470; Clontech) and pCMV-Myc vector. ..

    Polymerase Chain Reaction:

    Article Title: KCTD1 stabilizes c-Myc to upregulate PD-L1 and suppress anti-tumor immunity in hepatocellular carcinoma.
    Article Snippet: .. Full-length and truncated human c-Myc fragments including N-terminal (1–143 aa), central (144– 354 aa), and C-terminal (355–439 aa) were PCR-amplified and cloned into the pCMV-Myc vector (Clontech). ..

    Article Title: KCTD1 stabilizes c-Myc to upregulate PD-L1 and suppress anti-tumor immunity in hepatocellular carcinoma
    Article Snippet: .. Full-length and truncated human c-Myc fragments including N-terminal (1–143 aa), central (144–354 aa), and C-terminal (355–439 aa) were PCR-amplified and cloned into the pCMV-Myc vector (Clontech). ..

    Article Title: A selective RPL15 PROTAC degrader enhances anti-PD-1 immunotherapy in a murine melanoma tumor model
    Article Snippet: The following antibodies were used for immunoblotting: anti-Myc antibody (M4439, 9E10, ×1000 dilution, Sigma), anti-HA antibody (#3724, C29F4, ×1000 dilution, Cell Signaling Technology, Danvers, MA, USA), anti-FLAG antibody (F3165, M2, ×1000 dilution, Sigma), anti-CRBN (#71810, D8H3S, ×1000 dilution, CST), anti-Histone H3 (sc-517576, 1G1, ×500 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), anti-β-actin antibody (sc-69879, AC-15, ×2000 dilution, Santa Cruz Biotechnology), anti-eIF2α antibody (#9722, ×1000 dilution,Cell Signaling Technology), anti-phospho-eIF2α S51 antibody (#3398, D9G8, ×1000 dilution, Cell Signaling Technology), anti-JNK1/2 antibody (#9252, ×1000 dilution, Cell Signaling Technology), anti-phospho-JNK1/2 antibody T183/Y185 (#9251, ×1000 dilution, Cell Signaling Technology), anti-ZAK antibody (A301-994A, ×1000 dilution, Bethyl Laboratories, Waltham, MA, USA), anti-RPL15 antibody (VPA00489KT, ×1000 dilution, Bio-Rad Laboratories, Hercules, CA, USA), anti-RPS3 antibody (#9538, D50G7, ×1000 dilution, CST), anti-p53 antibody (sc-6243, ×500 dilution, Santacruz), anti-p21 antibody (F-5, sc-6246, ×300 dilution, Santacruz), anti-TOP1 antibody (EPR5375, ×1000 dilution, Abcam, Cambridge, UK), anti-mouse IgG-HRP antibody (NA931, ×9000 dilution, Cytiva, Tokyo, Japan), and anti-rabbit IgG-HRP antibody (NA934, ×9000 dilution, Cytiva). .. RPL15 cDNA was amplified from HeLa cells' cDNA by PCR and inserted into the pCMV-Myc vector (Takara Bio Inc., Shiga, Japan). .. For the construction of the lentiviral expression vector of human CRBN, Flag-tagged CRBN cDNA was amplified from HeLa cells' cDNA by PCR using the primer pairs containing FLAG sequences and inserted into the plenti-CAG-IRES-EGFP vector (#69047, Addgene).

    Ligation:

    Article Title: Parkin-mediated ubiquitination of hif-α modulates hypoxia signaling in Megalobrama amblycephala.
    Article Snippet: .. Following ligation of the amplified parkin gene fragment into the pCMV-Flag and pCMV-Myc vector (Clontech), the Flag-Ma-parkin and Myc-Ma-parkin fusion protein expression systems were constructed. .. AR TIC LE IN PR ES S 2.5 Quantitative real-time PCR (qRT-PCR) Analysis Phenol/chloroform-based RNA extraction from biological samples used TransZol reagent (TransGen Biotech).

    Article Title: Parkin-mediated ubiquitination of hif-α modulates hypoxia signaling in Megalobrama amblycephala
    Article Snippet: .. Following ligation of the amplified parkin gene fragment into the pCMV-Flag and pCMV-Myc vector (Clontech), the Flag- Ma-parkin and Myc- Ma-parkin fusion protein expression systems were constructed. .. Phenol/chloroform-based RNA extraction from biological samples used TransZol reagent (TransGen Biotech).

    Amplification:

    Article Title: Parkin-mediated ubiquitination of hif-α modulates hypoxia signaling in Megalobrama amblycephala.
    Article Snippet: .. Following ligation of the amplified parkin gene fragment into the pCMV-Flag and pCMV-Myc vector (Clontech), the Flag-Ma-parkin and Myc-Ma-parkin fusion protein expression systems were constructed. .. AR TIC LE IN PR ES S 2.5 Quantitative real-time PCR (qRT-PCR) Analysis Phenol/chloroform-based RNA extraction from biological samples used TransZol reagent (TransGen Biotech).

    Article Title: A selective RPL15 PROTAC degrader enhances anti-PD-1 immunotherapy in a murine melanoma tumor model
    Article Snippet: The following antibodies were used for immunoblotting: anti-Myc antibody (M4439, 9E10, ×1000 dilution, Sigma), anti-HA antibody (#3724, C29F4, ×1000 dilution, Cell Signaling Technology, Danvers, MA, USA), anti-FLAG antibody (F3165, M2, ×1000 dilution, Sigma), anti-CRBN (#71810, D8H3S, ×1000 dilution, CST), anti-Histone H3 (sc-517576, 1G1, ×500 dilution, Santa Cruz Biotechnology, Dallas, TX, USA), anti-β-actin antibody (sc-69879, AC-15, ×2000 dilution, Santa Cruz Biotechnology), anti-eIF2α antibody (#9722, ×1000 dilution,Cell Signaling Technology), anti-phospho-eIF2α S51 antibody (#3398, D9G8, ×1000 dilution, Cell Signaling Technology), anti-JNK1/2 antibody (#9252, ×1000 dilution, Cell Signaling Technology), anti-phospho-JNK1/2 antibody T183/Y185 (#9251, ×1000 dilution, Cell Signaling Technology), anti-ZAK antibody (A301-994A, ×1000 dilution, Bethyl Laboratories, Waltham, MA, USA), anti-RPL15 antibody (VPA00489KT, ×1000 dilution, Bio-Rad Laboratories, Hercules, CA, USA), anti-RPS3 antibody (#9538, D50G7, ×1000 dilution, CST), anti-p53 antibody (sc-6243, ×500 dilution, Santacruz), anti-p21 antibody (F-5, sc-6246, ×300 dilution, Santacruz), anti-TOP1 antibody (EPR5375, ×1000 dilution, Abcam, Cambridge, UK), anti-mouse IgG-HRP antibody (NA931, ×9000 dilution, Cytiva, Tokyo, Japan), and anti-rabbit IgG-HRP antibody (NA934, ×9000 dilution, Cytiva). .. RPL15 cDNA was amplified from HeLa cells' cDNA by PCR and inserted into the pCMV-Myc vector (Takara Bio Inc., Shiga, Japan). .. For the construction of the lentiviral expression vector of human CRBN, Flag-tagged CRBN cDNA was amplified from HeLa cells' cDNA by PCR using the primer pairs containing FLAG sequences and inserted into the plenti-CAG-IRES-EGFP vector (#69047, Addgene).

    Article Title: Parkin-mediated ubiquitination of hif-α modulates hypoxia signaling in Megalobrama amblycephala
    Article Snippet: .. Following ligation of the amplified parkin gene fragment into the pCMV-Flag and pCMV-Myc vector (Clontech), the Flag- Ma-parkin and Myc- Ma-parkin fusion protein expression systems were constructed. .. Phenol/chloroform-based RNA extraction from biological samples used TransZol reagent (TransGen Biotech).

    Article Title: Seneca Valley virus 2B and 3 C proteins attenuate the cGAS-STING signaling pathway by targeting STING for degradation.
    Article Snippet: Seneca Valley virus (SVV), an emerging porcine picornavirus, has attracted significant attention recently as it presents a substantial threat to the global pork industry.. Stimulator of interferon genes (STING) is an endoplasmic reticulum (ER)-associated adaptor protein that plays a crucial role in the type I interferon (IFN-I) signaling pathway, which triggers IFN-stimulated gene (ISG) production to elicit antiviral innate immunity.. SVV has evolved numerous strategies to counteract the IFN-I signaling pathway and evade innate immunity.

    Construct:

    Article Title: Parkin-mediated ubiquitination of hif-α modulates hypoxia signaling in Megalobrama amblycephala.
    Article Snippet: .. Following ligation of the amplified parkin gene fragment into the pCMV-Flag and pCMV-Myc vector (Clontech), the Flag-Ma-parkin and Myc-Ma-parkin fusion protein expression systems were constructed. .. AR TIC LE IN PR ES S 2.5 Quantitative real-time PCR (qRT-PCR) Analysis Phenol/chloroform-based RNA extraction from biological samples used TransZol reagent (TransGen Biotech).

    Article Title: Parkin-mediated ubiquitination of hif-α modulates hypoxia signaling in Megalobrama amblycephala
    Article Snippet: .. Following ligation of the amplified parkin gene fragment into the pCMV-Flag and pCMV-Myc vector (Clontech), the Flag- Ma-parkin and Myc- Ma-parkin fusion protein expression systems were constructed. .. Phenol/chloroform-based RNA extraction from biological samples used TransZol reagent (TransGen Biotech).

    Article Title: Seneca Valley virus 2B and 3 C proteins attenuate the cGAS-STING signaling pathway by targeting STING for degradation.
    Article Snippet: Seneca Valley virus (SVV), an emerging porcine picornavirus, has attracted significant attention recently as it presents a substantial threat to the global pork industry.. Stimulator of interferon genes (STING) is an endoplasmic reticulum (ER)-associated adaptor protein that plays a crucial role in the type I interferon (IFN-I) signaling pathway, which triggers IFN-stimulated gene (ISG) production to elicit antiviral innate immunity.. SVV has evolved numerous strategies to counteract the IFN-I signaling pathway and evade innate immunity.



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